Extraction Theory and General Procedure (Adapted from Mohrig, pp. These are often carried out using a separating funnel, as illustrated below.

Both DNA extraction and DNA quantification are crucial steps in forensic casework to obtain reliable and quality results. Liquid–liquid extraction (LLE), also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water (polar) and an organic solvent (non-polar). Compounds which are poorly miscible in organic solvents but highly miscible in water can be extracted into organic compounds either by (1) repetitive extraction with a separatory funnel or (2) by using a liquid-Liquid extractor. Solvent Extraction: Solvent Extraction is a separation method in which a solution (usually aqueous) comes into contact with a second solvent (usually organic) that is immiscible with the first to pass the solvent into the second solvent.. The main features that are sought in DNA extraction methods include high recovery of DNA, removal of impurities and inhibitors, and high–throughput processing. A. Alonso, in Encyclopedia of Forensic Sciences (Second Edition), 2013. There is a net transfer of one or more species from one liquid into another liquid phase, generally from aqueous to organic. Liquid-Liquid Extractor. Abstract.

Extraction methods … Organic chemistry employs solid-liquid, liquid-liquid, and acid-base extractions.

Laboratory Extractions Typical lab extractions are to transport organic compounds out of an aqueous phase and into an organic phase. The distribution of a solute between two phases is an equilibrium described by partition theory. 57-64, 72-77.) This occurs through the partitioning process, which involves the distribution of a solute between two immiscible liquid phases.

Extraction is a very common laboratory procedure used when isolating or purifying a product. https://www.khanacademy.org/.../separations-purifications/v/extractions

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